Project of the Scientific Grant Agency VEGA 2/0160/10

Project duration: 01/2010 – 12/2012

Principal investigator: Ing. Ján Maňka, CSc.

Deputy principal investigator: RNDr. Alexander Cigáň, CSc.

Deputy principal investigator: RNDr. Martin Kopáni, PhD. (Faculty of Medicine, Comenius University, Bratislava)

Financial support:

Aims of the project

Use of contactless SQUID magnetometric methods and magnetic resonance in application and evaluation of impacts of the gene therapy with the help of covered magnetic nanoparticles in the specific kidney diseases. Optimalization of the covered nanoparticles regarding the biocompatibility, stability and the transport properties inorder to enable their concentration (with coupled chemical) in the investigated organ by the magnetic focusation. Analysis of the efficiency of transfection of coupled gene into the cells by the detection of the red fluorescent protein in vivo at rats and by studying the structure and ultrastructure of the cells after the transfection in vitro. Design and realization of the magnetometric and MRI methods for detection of the concentration, transport, accumulation and clearing covered nanoparticles from the samples, models and the organs of trial animals.