{"id":4037,"date":"2025-12-10T14:21:51","date_gmt":"2025-12-10T13:21:51","guid":{"rendered":"https:\/\/www.um.sav.sk\/en\/?p=4037"},"modified":"2025-12-10T14:22:10","modified_gmt":"2025-12-10T13:22:10","slug":"1-method-for-quantifying-intramuscular-phosphocreatine-pcr-depletion-and-recovery-using-%c2%b3%c2%b9p-mrs-and-the-behavior-of-the-creatine-ch%e2%82%82-signal-in-%c2%b9h-mrs","status":"publish","type":"post","link":"https:\/\/www.um.sav.sk\/en\/selected-results\/1-method-for-quantifying-intramuscular-phosphocreatine-pcr-depletion-and-recovery-using-%c2%b3%c2%b9p-mrs-and-the-behavior-of-the-creatine-ch%e2%82%82-signal-in-%c2%b9h-mrs\/","title":{"rendered":"Method for quantifying intramuscular phosphocreatine (PCr) depletion and recovery using \u00b3\u00b9P MRS and the behavior of the creatine CH\u2082 signal in \u00b9H MRS"},"content":{"rendered":"

Investigator:<\/strong> Radka Klepochov\u00e1<\/p>\n

In this work, we focused on obtaining information about two key processes at the same time: the depletion and recovery of intramuscular phosphocreatine (PCr) using \u00b3\u00b9P MRS and the behavior of the creatine CH\u2082 signal in \u00b9H MRS, which exhibits similar dynamics. While previous studies required separate \u00b9H and \u00b3\u00b9P measurements with repeated loading, which complicated interpretation, we obtained both types of data for the first time in a single time-resolved interleaved experiment. This approach allowed us to directly compare the kinetics of Cr-CH\u2082 and PCr during exercise and recovery. We found a very close match between the time constants \u03c4PCr and \u03c4Cr-CH\u2082, the expected differences between groups with lower and higher BMI were preserved, and we observed a positive correlation between the rate of signal decline. Our results suggest that under certain conditions, the \u00b9H MRS signal of creatine can be used as a substitute indicator of oxidative capacity, traditionally determined using \u00b3\u00b9P MRS. Based on these findings, we published an article in the Q1 journal NMR in Biomedicine.<\/p>\n

\"\"<\/p>\n

Fig. 1: Representative time course of 1H (top) and 31P (bottom) spectra obtained during 3 minutes of rest, 5 minutes of plantar flexion exercise, and 10 minutes of recovery from the calf muscle of one volunteer. Protons and phosphorus, which cause the MRS signal, are highlighted in the structural patterns (P)Cr.<\/em><\/p>\n

Publications:<\/strong><\/p>\n

KLEPOCHOV\u00c1, Radka<\/u> (73%) \u2013 NIESS, F. (2%) \u2013 METZ, M. (2%) \u2013 UKROPCOV\u00c1, B. (2%) \u2013 UKROPEC, J. (2%) \u2013 TRATTNIG, S. (2%) – KAUTZKY-WILLER, A. (2%) \u2013 KR\u0160\u0160\u00c1K, M. (5%) \u2013 MEYERSPEER, M. (10%) 1<\/sup>H and 31<\/sup>P MRS interleaved with high time resolution reveals closely matching creatine CH\u2082 and PCr dynamics during exercise. In NMR in Biomedicine<\/em>, 2025, vol. 38, no. 10, art. no. e70132. ISSN 0952-3480. (2024: 2.7 – IF, Q1 – JCR, 0.95 – SJR, Q1 – SJR) https:\/\/doi.org\/10.1002\/nbm.70132<\/em><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Investigator: Radka Klepochov\u00e1 In this work, we focused on obtaining information about two key processes at the same time: the depletion and recovery of intramuscular…<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[33,37,30],"tags":[],"class_list":["post-4037","post","type-post","status-publish","format-standard","hentry","category-international-projects","category-mri","category-selected-results"],"publishpress_future_action":{"enabled":false,"date":"2026-04-22 16:38:16","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category","extraData":[]},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/www.um.sav.sk\/en\/wp-json\/wp\/v2\/posts\/4037","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.um.sav.sk\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.um.sav.sk\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.um.sav.sk\/en\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/www.um.sav.sk\/en\/wp-json\/wp\/v2\/comments?post=4037"}],"version-history":[{"count":1,"href":"https:\/\/www.um.sav.sk\/en\/wp-json\/wp\/v2\/posts\/4037\/revisions"}],"predecessor-version":[{"id":4038,"href":"https:\/\/www.um.sav.sk\/en\/wp-json\/wp\/v2\/posts\/4037\/revisions\/4038"}],"wp:attachment":[{"href":"https:\/\/www.um.sav.sk\/en\/wp-json\/wp\/v2\/media?parent=4037"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.um.sav.sk\/en\/wp-json\/wp\/v2\/categories?post=4037"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.um.sav.sk\/en\/wp-json\/wp\/v2\/tags?post=4037"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}